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KMID : 0545120140240050585
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 5 p.585 ~ p.591
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Degradation of Lignocelluloses in Rice Straw by BMC-9, a Composite Microbial
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Hongyan Zhao
Hairu Yu
Xufeng Yuan
Renzhe Piao
Hulin Li
Xiaofen Wang
Zongjun Cui
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Abstract
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To evaluate the potential utility of pretreatment of raw biomass with a complex microbial system, we investigated the degradation of rice straw by BMC-9, a lignocellulose decomposition strain obtained from a biogas slurry compost environment. The degradation characteristics and corresponding changes in the bacterial community were assessed. The results showed that rapid degradation occurred from day 0 to day 9, with a peak total biomass bacterium concentration of 3.3 × 108 copies/ml on day 1. The pH of the fermentation broth declined initially and then increased, and the mass of rice straw decreased steadily. The highest concentrations of volatile fatty acid contents (0.291 mg/l lactic acid, 0.31 mg/l formic acid, 1.93 mg/l acetic acid, and 0.73 mg/l propionic acid) as well as the highest xylanse activity (1.79 U/ml) and carboxymethyl cellulase activity (0.37 U/ml) occurred on day 9. The greatest diversity among the microbial community also occurred on day 9, with the presence of bacteria belonging to Clostridium sp., Bacillus sp., and Geobacillus sp. Together, our results indicate that BMC-9 has a strong ability to rapidly degrade the lignocelluloses of rice straw under relatively inexpensive conditions, and the optimum fermentation time is 9 days.
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KEYWORD
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microbial community, rice straw degradation, qPCR, volatile fatty acids
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